Plant Transcription Factor Database
Previous version: v3.0
Oryza glaberrima
FAR1 Family
Species TF ID Description
ORGLA02G0073800.1FAR1 family protein
ORGLA02G0111800.1FAR1 family protein
ORGLA02G0153200.1FAR1 family protein
ORGLA02G0169200.1FAR1 family protein
ORGLA02G0172700.1FAR1 family protein
ORGLA02G0203300.1FAR1 family protein
ORGLA02G0203500.1FAR1 family protein
ORGLA03G0057700.1FAR1 family protein
ORGLA03G0090000.1FAR1 family protein
ORGLA03G0105900.1FAR1 family protein
ORGLA03G0259500.1FAR1 family protein
ORGLA03G0331000.1FAR1 family protein
ORGLA03G0374400.1FAR1 family protein
ORGLA03G0393200.1FAR1 family protein
ORGLA04G0094900.1FAR1 family protein
ORGLA04G0107700.1FAR1 family protein
ORGLA04G0130500.1FAR1 family protein
ORGLA04G0223700.1FAR1 family protein
ORGLA05G0035000.1FAR1 family protein
ORGLA05G0097200.1FAR1 family protein
ORGLA06G0040800.1FAR1 family protein
ORGLA06G0166100.1FAR1 family protein
ORGLA06G0227100.1FAR1 family protein
ORGLA06G0284700.1FAR1 family protein
ORGLA07G0068100.1FAR1 family protein
ORGLA07G0123500.1FAR1 family protein
ORGLA07G0123700.1FAR1 family protein
ORGLA07G0152100.1FAR1 family protein
ORGLA07G0170400.1FAR1 family protein
ORGLA08G0021700.1FAR1 family protein
ORGLA10G0024000.1FAR1 family protein
ORGLA11G0071600.1FAR1 family protein
ORGLA11G0090200.1FAR1 family protein
ORGLA12G0035900.1FAR1 family protein
ORGLA12G0060700.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885